December 16, 2024

PROTEO Laval

Journée scientifique PROTEO Laval

About the PROTEO Symposium

Welcome to the 22nd edition of our annual symposium!

It is with great pleasure that PROTEO invites you to its annual symposium, which will be held on Thursday, May 11 and Friday, May 12 at the Théâtre de la Cité Universitaire (TCU), Palasis-Prince Hall, Laval University.

This event is an excellent opportunity for all PROTEO members to learn more about the diversity of our expertise, our research interests and to thus pave the way for new collaborations.

POSTER AND ORAL PRESENTATIONS :

The deadline to submit your abstract for a poster presentation is April 15, 2023 at 5:00 PM.

Details are available under the "Instructions" tab.

STUDENT/POSTDOC LECTURES:

This year, several students (including the Michèle-Auger award winner) will be selected to present their research project orally in short lectures. Interested students must submit their abstract (submission tab) by April 6, 2023, specifying their willingness to make an oral presentation. The selection committee will choose the students and they will be notified before the end of April.

We hope to see many of you there!

About the PROTEO Symposium

Kimberley Reynolds

Title: The Genetic Landscape of a Biochemical Interaction

Abstract: Individual proteins can be expressed, purified, and exquisitely characterized in terms of their biochemical and biophysical parameters in vitro. However, the quantitative relationship between these parameters and complex phenotypes like growth remains mysterious. For example, what values of protein abundance, thermal stability (ΔGfold) and catalytic activity (kcat, Km) must an enzyme attain to sustain metabolic pathway flux and support cell growth? In many cases, we are missing even orders-of-magnitude level bounds on these fundamental biochemical parameters — we do not have a sense of which protein properties must be precisely tuned and which are robust to variation. To address this knowledge gap, my lab seeks to quantify the intracellular constraints on protein abundance, activity, regulation, and ultimately sequence. We then use this information to engineer new protein systems and build mathematical models relating protein activity and sequence to phenotype. In this talk, I will discuss our recent study of how variation in the activity of one enzyme constrains the biochemical parameters and sequence of another. Using a combination of deep mutational scanning and mathematical modeling we showed that inter-enzyme biochemical coupling can strongly reshape an enzyme’s sensitivity to mutation. Our data provide a comprehensive view of sequence constraints in a biochemically linked enzyme pair and open the door to high throughput measurements of enzyme biochemical parameters using growth-based assays.

Kimberley Reynolds

À propos

PROTEO Laval est un centre de recherche reconnu par la Commission de la recherche de l'Université Laval. Il regroupe 24 membres chercheurs de différentes facultés : Faculté des sciences et de génie, Faculté de médecine et Faculté de pharmacie.

Location

Théâtre de la Cité Universitaire, Palasis-Prince, ULaval

2305 Rue de l'Université Québec, QC Canada, G1V 0A6

Registration period

October 21, 2024 - 00:00 until December 2, 2024 - 09:00

Submission period

October 21, 2024 - 07:00 until November 29, 2024 - 23:59

Contact us

If you have any questions, please contact coordination@proteo.ulaval.ca .

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